4 F eb 2 01 4 Multiple Lac - mediated loops revealed by Bayesian statistics and tethered particle motion
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چکیده
The bacterial transcription factor LacI loops DNA by binding to two separate locations on the DNA simultaneously. Despite being one of the best-studied model systems for transcriptional regulation, the number and conformations of loop structures accessible to LacI remain unclear, though the importance of multiple coexisting loops has been implicated in interactions between LacI and other cellular regulators of gene expression. To probe this issue, we have developed a new analysis method for tethered particle motion (TPM), a versatile and commonlyused in vitro single-molecule technique. Our method, vbTPM, is based on a varia-
منابع مشابه
Multiple LacI-mediated loops revealed by Bayesian statistics and tethered particle motion
The bacterial transcription factor LacI loops DNA by binding to two separate locations on the DNA simultaneously. Despite being one of the best-studied model systems for transcriptional regulation, the number and conformations of loop structures accessible to LacI remain unclear, though the importance of multiple coexisting loops has been implicated in interactions between LacI and other cellul...
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تاریخ انتشار 2014